產(chǎn)品說(shuō)明書
FAQ
COA
已發(fā)表文獻(xiàn)
性能參數(shù)
|
分子別名（Synonyms） |
IgG1; VH7183; RGD1359539; |
|
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source） |
Mouse IgG1 Fc Protein is expressed from HEK293 without tag. It contains Val98-Lys324.[Accession | P01868] |
|
分子量大?。∕olecular Weight） |
The protein has a predicted MW of 25.7 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result. |
|
內(nèi)毒素（Endotoxin） |
Less than 1EU per μg by the LAL method. |
|
純度（Purity） |
> 95% as determined by SDS-PAGE and HPLC. |
|
活性（Activity） |
SPR Data: Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IgG1 Fc, No Tag with an affinity constant of 15.09 nM as determined in SPR assay . |
|
制劑（Formulation） |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
重構(gòu)方法（Reconstitution） |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
儲(chǔ)存條件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事項(xiàng)
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
