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            Recombinant Human APOE4/Apolipoprotein E Protein,HisTag

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            產(chǎn)品說明書

            FAQ

            COA

            已發(fā)表文獻(xiàn)

            性能參數(shù)

            蛋白別名(Synonyms)

            Apo-E;Apolipoprotein E; APOE

            表達(dá)系統(tǒng)及表達(dá)區(qū)間(Source)

            Human APOE4/Apolipoprotein E Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Lys19-His317.[Accession | AAB59397.1]

            分子量(Molecular Weight)

            The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on Tris-Bis PAGE result.

            內(nèi)毒素(Endotoxin)

            Less than 1EU per μg by the LAL method.

            純度(Purity)

            > 95% as determined by Tris-Bis PAGE

            制劑(Formulation)

            Lyophilized from 0.22μm filtered solution in PBS, 2mM DTT, 5mM CHAPS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

            復(fù)溶方法(Reconstitution)

            Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

             

            儲存條件

            The product should be stored at -25~-15℃ for 1 year from date of receipt.

            2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

            3-6 months, -85~-65℃ under sterile conditions after reconstitution.

            Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

             

            注意事項

            1. Please operate with lab coats and disposable gloves,for your safety.       
            2. This product is for research use only.         

             

            Ver. CN20231201

            400-6111-883