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            Recombinant Human TGF-beta RII/TGFBR2 Protein,hFc Tag

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            產(chǎn)品說(shuō)明書(shū)

            FAQ

            COA

            已發(fā)表文獻(xiàn)

            性能參數(shù)

            表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source

            Human TGF-beta RII/TGFBR2 Protein is expressed from HEK293 with hFc tag at the N-terminal. It contains Ile24-Asp159.[Accession | P37173-1]

            分子量大小(Molecular Weight

            The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 50-68 kDa based on Tris-Bis PAGE result.

            內(nèi)毒素(Endotoxin

            Less than 1EU per μg by the LAL method.

            純度(Purity

            > 95% as determined by Tris-Bis PAGE and HPLC

            活性(Activity

            ELISA Data: Immobilized Human Mature TGF beta 1 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 23.2ng/ml determined by ELISA. 

            制劑(Formulation

            Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

            重構(gòu)方法(Reconstitution

            Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

             

            儲(chǔ)存條件

            The product should be stored at -25~-15℃ for 1 year from date of receipt.

            2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

            3 months, -25~-15℃ under sterile conditions after reconstitution.

            Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

             

            注意事項(xiàng)

            1.Please operate with lab coats and disposable gloves,for your safety.

            2.This product is for research use only.

             

            Ver. CN20230625

            400-6111-883