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產(chǎn)品信息
|
產(chǎn)品名稱 |
產(chǎn)品編號 |
規(guī)格 |
|
Recombinant Human/Mouse Wnt-5a Protein, His Tag 重組人/小鼠 Wnt5a蛋白 |
92282ES10 |
10 μg |
|
92282ES60 |
100 μg |
|
|
92282ES76 |
500 μg |
性能參數(shù)
|
別名（Synonyms） |
Wingless-type MMTV Integration Site Family, Member 5a; wingless-type MMTV integration site family, member 5A; Wnt5a |
|
表達(dá)系統(tǒng)（Source） |
HEK293 Cells |
|
表達(dá)序列（Sequence） |
Gln38-Lys380 |
|
Accession |
P41221 |
|
分子量大?。?/font>Molecular Weight） |
The protein has a predicted MW of 40kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation |
|
純度（Purity） |
> 90% by SDS-PAGE. |
|
內(nèi)毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
生物活性（Biological Activity） |
Measured by its ability to inhibit Wnt-3a-induced a TCF/LEF luciferase reporter expression by 293F cells. The ED50 for this effect is 2-10µg/mL. Optimal concentrations should be determined by each laboratory for each application |
|
制劑（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and 0.01 % Tween 80 were added as protectant before lyophilization |
|
標(biāo)簽（Tag） |
His tag at the C-terminal |
|
外觀（Physical Appearance） |
凍干粉 |
|
Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
儲存條件
-85~-65℃保存，收到貨之后有效期1年。
避免反復(fù)凍融，打開小瓶前先離心。建議等分分裝為一次實驗使用量，分裝后，-80℃凍存。
復(fù)溶方法
開蓋前，先離心。在含有至少0.1%人或牛血清白蛋白的無菌PBS中復(fù)溶，復(fù)溶到100μg/mL的濃度。
注意事項
- 為了您的安全和健康，請穿實驗服并戴一次性手套操作。
- 本產(chǎn)品僅作科研用途。
Ver.CN20240909
