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            Recombinant Cynomolgus Fc gamma RIIB Protein,His Tag

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            產(chǎn)品說明書

            FAQ

            COA

            已發(fā)表文獻(xiàn)

            性能參數(shù)

            表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source

            Cynomolgus Fc gamma RIIB Protein is expressed from HEK293 with His tag at the C-terminal. It contains Ala46-Pro224.[Accession | Q8SPW3]

            分子量大?。?/font>Molecular Weight

            The protein has a predicted MW of 21 kDa. Due to glycosylation, the protein migrates to 33-40 kDa based on Tris-Bis PAGE result.

            內(nèi)毒素(Endotoxin

            Less than 1EU per μg by the LAL method.

            純度(Purity

            > 95% as determined by Tris-Bis PAGE
            > 95% as determined by HPLC

            活性(Activity

            SPR Data: Trastuzumab captured on CM5 Chip via Protein A can bind Cynomolgus Fc gamma RIIB, His Tag with an affinity constant of 2.72 μM as determined in SPR assay (Biacore T200).

            制劑(Formulation

            Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

            重構(gòu)方法(Reconstitution

            Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

             

            儲存條件

            The product should be stored at -25~-15℃ for 1 year from date of receipt.

            2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

            3 months, -25~-15℃ under sterile conditions after reconstitution.

            Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

             

            注意事項

            1.Please operate with lab coats and disposable gloves,for your safety.

            2.This product is for research use only.           

            Ver. CN20230620

            400-6111-883