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            Mouse EG-VEGF

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            產(chǎn)品說明書

            FAQ

            COA

            已發(fā)表文獻

            Endocrine gland-derived vascular endothelial growth factor (EG-VEGF), also called prokineticin 1 (PK1), is a member of the prokineticin family of secreted proteins that share a common structural motif containing ten conserved cysteine residues that form five pairs of disulfide bonds. The mature region in mouse is 93% and 87% aa identical to the mature regions in rat and human. Mouse EGVEGF stimulates proliferation and survival of liver sinusoidal endothelial cells, and, in mouse, EG-VEGF acts to induce monocyte migration and stimulate hematopoiesis.

             

            Product Properties

            Synonyms

            EGVEGF, Mambakine, PROK1, Prokineticin 1

            Accession

            Q14A28

            GeneID

            246691

            Source

            E.coli-derived mouse EG-VEGF protein, Ala20-Phe105.

            Molecular Weight

            Approximately 9.6 kDa.

            AA Sequence

            AVITGACERD IQCGAGTCCA ISLWLRGLRL CTPLGREGEE CHPGSHKIPF LRKRQHHTCP CSPSLLCSRF PDGRYRCFRD LKNANF

            Tag

            None

            Physical Appearance

            Sterile Filtered White lyophilized (freeze-dried) powder.

            Purity

            >95% by SDS-PAGE and HPLC analyses.

            Biological Activity

            The ED50 as Measured in a cell proliferation assay using EJG bovine adrenal-derived endothelial cells. Fully biologically active when compared to standard.

            Endotoxin

            < 0.1 EU per 1μg of the protein by the LAL method.

            Formulation

            Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4, with 3% Trehalose.

            Reconstitution

            We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20. Further dilutions should be made in appropriate buffered solutions.

             

            Shipping and Storage

            The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

            Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

             

            Cautions

            1. Avoid repeated freeze-thaw cycles.

            2. For your safety and health, please wear lab coats and disposable gloves for operation.

            3.For research use only!

             

            HB220520

            400-6111-883