Synonyms

R-Spondin 1;Roof Plate-specific Spondin 1；Cristin3; R-spondin-1

Source

Expressed in E. coli.

Molecular Weight

Approximately 20.9kDa, a single non-glycosylated polypeptide chain containing 189 amino acid

Accession

Q9Z132: Ser21-Gly209

Biological Activity

Fully biologically active when compared to standard. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED₅₀ is 50-200 ng/mL in the presence of 5ng/mL recombinant mouse Wnt-3a.

Purity

> 96% by SDS-PAGE.

Endotoxin

＜0.01EU/μg protein as determined by LAL method.

Formulation

Lyophilized from a 0.2mm filtered solution in 20mM PB, 150mM NaCl, pH 7.4.

Reconstitution

Before use this product, please read the direction below carefully.

1.This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.