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            Recombinant Human HCC-1/CCL14 重組人HCC-1/CCL14
             
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            產(chǎn)品說明書
                  
            FAQ
                  
            COA
                  
            已發(fā)表文獻(xiàn)
                  
             
            Human CCL14 is belonging to the CC chemokine family. It has two isoforms, CCL14a (HCC-1) and CCL14b (HCC-3). The sequence of HCC-3 differs from HCC-1 as follow: 27-27 R→ QTGGKPKVVKIQLKLVG. CCL14 was first isolated from the hemofiltrate of human patients with chronic renal failure. CCL14 promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1 and is a ligand for CCR1, CCR3 and CCR5. CCL14 is expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations in plasma.
            


             
            


            Product Properties
            


            
	
		
            
			
			
            Synonyms
            
			            		
			
			
            CC-1 Protein, Human; CC-3 Protein, Human; CKB1 Protein, Human; FLJ16015 Protein, Human; HCC-1 Protein, Human; HCC-1(1-74) Protein, Human; HCC-1/HCC-3 Protein, Human; HCC-3 Protein, Human; MCIF Protein, Human; NCC-2 Protein, Human; NCC2 Protein, Human; SCYA14 Protein, Human; SCYL2 Protein, Human; SY14 Protein, Human
            
			            		
		
            
		
            
			
			
            Accession
            
			            		
			
			
            Q16627
            
			            		
		
            
		
            
			
			
            GeneID
            
			            		
			
			
            6358
            
			            		
		
            
		
            
			
			
            Source
            
			            		
			
			
            E.coli-derived human Hemofiltrate CC Chemokine-1 Protein, Thr22-Asn93
            
			            		
		
            
		
            
			
			
            Molecular Weight
            
			            		
			
			
            Approximately 8.4 kDa.
            
			            		
		
            
		
            
			
			
            AA Sequence
            
			            		
			
			
            TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN
            
			            		
		
            
		
            
			
			
            Tag
            
			            		
			
			
            None
            
			            		
		
            
		
            
			
			
            Physical Appearance
            
			            		
			
			
            Sterile Filtered White lyophilized (freeze-dried) powder.
            
			            		
		
            
		
            
			
			
            Purity
            
			            		
			
			
            > 96 % by SDS-PAGE and HPLC analyses.
            
			            		
		
            
		
            
			
			
            Biological Activity
            
			            		
			
			
            Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-20 ng/ml.
            
			            		
		
            
		
            
			
			
            Endotoxin
            
			            		
			
			
            < 1.0 EU per 1μg of the protein by the LAL method.
            
			            		
		
            
		
            
			
			
            Formulation
            
			            		
			
			
            Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
            
			            		
		
            
		
            
			
			
            Reconstitution
            
			            		
			
			
            We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
            
			            		
		
            
	            

            


             
            


            Shipping and Storage
            


            The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
            


            Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
            


             
            


            Cautions
            


            1. Avoid repeated freeze-thaw cycles.
            


            2. For your safety and health, please wear lab coats and disposable gloves for operation.
            


            3. For research use only.
            


             
            


             
            


            HB220425 
            

               
             
             
             
             
             
             
             
            400-6111-883