產(chǎn)品說明書
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產(chǎn)品信息
|
產(chǎn)品名稱 |
產(chǎn)品編號 |
規(guī)格 |
|
Recombinant Human DKK-1 Protein, His Tag |
92275ES25 |
25 μg |
|
92275ES60 |
100 μg |
|
|
92275ES76 |
500 μg |
產(chǎn)品描述
Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes.
性能參數(shù)
|
別名（Synonyms） |
Dickkopf-1;DKK-1;SK Protein; DKK1; dkk1; dkk-1 |
|
表達區(qū)間及表達系統(tǒng)（Source） |
Human DKK1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Thr32-His266.[Accession | O94907] |
|
分子量大?。?/font>Molecular Weight） |
The protein has a predicted MW of 26.87 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result. |
|
內(nèi)毒素（Endotoxin） |
< 1.0 EU/μg by the LAL method. |
|
純度（Purity） |
> 95% as determined by Tris-Bis PAGE and HPLC |
|
生物活性（Biological Activity） |
ELISA Data: Immobilized Human DKK1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. |
|
制劑（Formulation） |
Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
外觀（Physical Appearance） |
白色凍干粉 |
儲存條件
-25~-15℃保存，收到貨之后有效期1年。
復溶后， 2 ~8 °C保存，7天有效期。復溶后， -85~-65℃保存，3個月有效期。
建議首次使用時將蛋白等分，避免重復凍融循環(huán)。
復溶方法
開蓋之前，先離心。使用去離子水復溶至濃度不低于0.1 mg/mL。
注意事項
- 為了您的安全和健康，請穿實驗服并戴一次性手套操作。
- 本產(chǎn)品僅作科研用途。
Ver. CN20240407
