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            Recombinant Human IL-3 GMP Protein 重組人白介素3

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            產(chǎn)品說明書

            FAQ

            COA

            已發(fā)表文獻(xiàn)

            產(chǎn)品簡介

             

            白細(xì)胞介素-3Interleukin-3,IL-3是一種造血生長因子,可促進(jìn)巨核細(xì)胞、粒細(xì)胞-巨噬細(xì)胞、紅系、嗜酸性粒細(xì)胞、嗜堿性粒細(xì)胞和肥大細(xì)胞譜系的定向祖細(xì)胞的存活、分化和增殖。IL-3由T細(xì)胞、肥大細(xì)胞和嗜酸性粒細(xì)胞產(chǎn)生,可增強(qiáng)血小板生成素、吞噬作用和抗體介導(dǎo)的細(xì)胞毒性。其激活單核細(xì)胞的能力表明IL-3可能具有額外的免疫調(diào)節(jié)作用。許多IL-3活性依賴于與其他細(xì)胞因子的共同刺激。

            重組人IL-3是一種15.0kDa的重組蛋白,含有133個(gè)氨基酸殘基。本品由大腸桿菌表達(dá),以凍干粉形式提供,高活性、高純度、低內(nèi)毒素、無標(biāo)簽。

             

            性能參數(shù)

             

            別名(Synonyms

            Interleukin-3; IL-3; Hematopoietic growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor

            表達(dá)系統(tǒng)(Source

            E.coli.

            表達(dá)序列(Sequence

            Ala20-Phe152.

            分子量(Molecular Weight

            Approximately 15.08 kDa.

            Accession

            P08700

            標(biāo)簽(Tag)

            No Tag

            生物活性(Biological Activity

            The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7 × 106 IU/mg.

            純度(Purity

            > 95% as determined by SDS-PAGE.

            內(nèi)毒素(Endotoxin

             0.01EU/μg protein as determined by LAL method.

            無菌(Sterility

            Negative.

            支原體檢測(cè)(Mycoplasma

            Negative.

            外源病毒(Vitro Virus

            Negative.

            宿主殘留蛋白(Host Cell Protein

            Less than 0.5 ng/ug when tested by ELISA.

            宿主殘留DNA(Host Cell DNA

            Less than 20 ng/mg when tested by qPCR.

            制劑(Formulation

            Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.

            外觀(Physical Appearance

            Sterile filtered white lyophilized (freeze-dried) powder.

             

            儲(chǔ)存條件

             

            -25~-15℃保存,有效期3年。

            復(fù)溶后, 2 ~8 保存,30天有效期。復(fù)溶后, -85~-65℃保存,3個(gè)月有效期。

             

            復(fù)溶方法

             

            建議將Human IL-3在無菌PBS中復(fù)溶至0.1-1.0 mg/ml的濃度。盡可能避免反復(fù)凍融。

             

            注意事項(xiàng)

             

            1.為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

            2.本產(chǎn)品僅作科研用途。

             

            產(chǎn)品數(shù)據(jù)

             

            SDS- PAGE

             

            Human IL-3 on SDS-PAGE under reduced condition.

            The purity is greater than 95%.

             

            Cell Based Assay

            The ED50 as determined by a cell proliferation assay using human TF-1 cells

             is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7 × 106 IU/mg.

             

            Ver. CN20241022

            400-6111-883