產(chǎn)品說明書
FAQ
COA
已發(fā)表文獻
性能參數(shù)
|
蛋白別名（Synonyms） |
ETGF; TGF-alpha; TGF type 1; TGFA |
|
種屬（Species) |
Human |
|
表達系統(tǒng)（Source） |
HEK293 |
|
表達序列（Sequence） |
Val40-Ala89 |
|
Accession |
P01135-1 |
|
標簽（Tag) |
N-hFc |
|
分子量（Molecular Weight） |
The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result. |
|
內(nèi)毒素（Endotoxin） |
Less than 1EU per μg by the LAL method. |
|
純度（Purity） |
> 95% as determined by Bis-Tris PAGE and HPLC |
|
活性（Activity） |
ELISA Data: Immobilized Human TGF-alpha, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA
SPR Data: Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 μM as determined in SPR assay. |
|
制劑（Formulation） |
Lyophilized from 0.22μm filtered solution in 50mM Tris,100mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
|
外觀（Appearance） |
凍干粉 |
儲存條件
-25~-15℃保存，收到貨之后有效期1年。復(fù)溶后， -85~-65℃保存，3個月有效期。
復(fù)溶方法
開蓋之前，先離心。建議將凍干的蛋白使用蒸餾水溶解，復(fù)溶濃度超過100μg/ml。
注意事項
- 為了您的安全和健康，請穿實驗服并戴一次性手套操作。
- 本產(chǎn)品僅作科研用途。
Ver. CN20241023
